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KMID : 0385520120250030171
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Analytical Science & Technology
2012 Volume.25 No. 3 p.171 ~ p.177
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Determination of aromatic amino acids by chemiluminometric assay with Luminol-H2O2-Cu(II) system
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Kim Kyung-Min
Kim Young-Ho
Lee Sang-Hak
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Abstract
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A determination method of aromatic amino acids such as trytophan (Trp), tyrosine (Tyr), and phenylalanine (Phe) using luminol-H2O2-Cu(II) system has been presented. In the presence of an aromatic amino acid, the enhanced chemiluminescence (CL) intensity of luminol-H2O2-Cu(II) system was obtained by forming a complex between Cu(II) and the amino acid. Based on the above phenomenon, a sensitive and fast determination of three aromatic amino acids was performed using the CL method in batch-type detection system. To optimize determination conditions, the kinetic influence of an aromatic amino acid on the luminol-H2O2-Cu(II) system and the effects of H2O2 and Cu(II) concentration, pH, and buffers were investigated. Under the optimized conditions, the calibration curve was linear over the range from 1.0 × 10?6
to 2.0 × 10?5 M for Trp, 1.0 × 10?6 to 2.0 × 10?5 M for Try, and 2.0 × 10?6 to 2.0 × 10?5 M for Phe, respectively. In this range, reproducibility (RSD, n = 4) of Trp, Try, and Phe were 3.21%, 2.64%, and 2.48%, respectively. The limit of detection (3σ/s) was calculated to be 6.8 × 10?7 M for Trp, 5.7 × 10?7 M for Try, and 9.6 × 10?7 M for Phe.
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KEYWORD
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luminol-H2O2-Cu(II), chemiluminescence, aromatic amino acid
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